Impact of Translighters on the Stability of Experiments

Research of Impact of IMCS* Structures (Translighters) on the Stability of Experiments Using Molecular Biology Methods

Kseniya Loshakova

Microbiological Monitoring and Biological Purification Laboratory

Genomic Research and Technology Department

All-Russia Research Institute for Agricultural Microbiology. St. Petersburg, Russia

• Microbial culture inoculation and growing in artificial media.
• Stable DNA purification from various materials (cultured microbial media, soils,agarose gel).
• Improving quality of electrophoresis reactions.
• Successful amplification and restriction by molecular genomic and physical methods. The study was performed using system adapters Translighters Super, TranslightersBlaga. Structural Impact on DNA Purification The impact of structures was evaluated in an experiment with the use of fulvic acids and glucose sent to our genomic technologies laboratory from a soil institute in the Netherlands.
  DNA purification from fulvic acid and glucose substrates has remained a difficult, laborious task. Nobody from European specialists was willing to take on this challenge. Having a well-equipped technical base, our laboratory decided to take the risk. Our attempts were repeatedly unsuccessful for two months. But in February 2014, we received the first positive results of this experiment. This success, as subsequent research has shown, can be clearly attributed to the influence of the structures (Translighter Super mainly and Translighter Blaga).
  Below are the results of DNA purification with structures and without (Fig. 1, Fig. 2, Fig. 3). Fig. 1 Raw DNA extract electrophoresis gel in agarose gel. Without the adapters.

Impact of Translighters on the Stability of Experiments